郁金散对大肠湿热证模型大鼠血清及肠道组织胃肠激素的影响

doi:10.11843/j.issn.0366-6964.2017.06.019

Abstract

Abstract:

This experiment was conducted to explore the effects of Yujin Powder on gastrointestinal hormone in rat model of serum, ileum and colon tissue of large intestine dampness-heat syndrome (LIDHS) rat model. Sixty Wistar rats (180-220 g) were randomly divided into 6 groups with 10 rats in each group: normal control group, model group of LIDHS, self-healing group, high, middle and low dose of Yujin Powder groups. The LIDHS model was established through the following steps: let them drink honey water freely; in the first 10 days, fasting and sufficient feed combined with gavage of lard were alternately performed; the rats were gavaged with wine and put in high temperature and high humidity environment (8 h·d^-1) during the following 5 days,and then they were intraperitoneally injected with Escherichia coli once again after 24 hours. After establishment of the model, the rats were treated with high (10.4 g·kg^-1), middle (5.2 g·kg^-1) and low-dose (2.6 g·kg^-1) Yujin Powder, respectively. During the experiment, the clinical symptoms and signs of rats were observed, weights were recorded and the contents of gastrointestinal hormone in serum and ileum and colon tissue were detected. Results were as follows: Model rats appeared loose stools. Their feces were yellow and rotten. The anus was red and swollen and so on. The weights of normal group were gradually increase. The weights of model rats showed a trend from fluctuating rise to gradually decline. After treatment with all doses of Yujin Powder, the weights gradually rose, especially in the high dose group, but no significant different was found. Compared with normal group, the contents of motilin (MTL), gastrin (GAS), substance P (SP) increased significantly (P<0.01 or P<0.05) in serum, ileum and colon tissue in model and self-healing groups. Somatostatin (SS) decreased significantly (P<0.01) and vasoactive intestinal peptide (VIP) showed no significant changes. Compared with self-healing group, after treatment with various doses of Yujin Powder, the above-mentioned indexes tend to normal condition to varying degrees, especially in the high dose group. In the high dose group, the contents of MTL, GAS and SP decreased significantly (P<0.01 or P<0.05), and SS increased significantly (P<0.01 or P<0.05) in serum and ileum and colon tissue. It was demonstrated that the gastrointestinal hormones presented disorder in LIDHS rat model, that the contents of MTL, GAS, SP and VIP significantly increased and SS declined in LIDHS rat model. Yujin Powder could significantly adjust the disorder state of gastrointestinal hormone in LIDHS model rat and the effects of high dose was the best.

CLC Number:

S853.9

WEN Yan-qiao, YAO Wan-ling, YANG Chao-xue, MA Qi, ZHANG Xiao-song, JI Peng, HUA Yong-li, WEI Yan-ming. Effects of Yujin Powder on Gastrointestinal Hormone in Serum and Intestinal Tissue of Large Intestine Dampness-heat Syndrome Rat Model[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1140-1149.

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Effects of Yujin Powder on Gastrointestinal Hormone in Serum and Intestinal Tissue of Large Intestine Dampness-heat Syndrome Rat Model

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